Browse all practice questions for the Ontario Grade 12 University Biology (SBI4U) Course Practice Exam. Search by topic, open any question and review its full explanation, then test yourself in the practice quiz.

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  • What is the primary role of the Golgi apparatus in a cell?
  • What is the main difference between glomerulus filtrate and blood plasma?
  • What occurs when the membrane potential is reduced to less than -70 mV?
  • Which structure in the kidney acts as a filtration device?
  • What are the three main types of neurons based on function?
  • What are the muscular tubes that transport urine from the kidneys to the bladder called?
  • Which pigment is essential for photosynthesis in plants?
  • What type of bonds are primarily found within a molecule?
  • During which process are ribonucleic acids synthesized from a DNA template?
  • Which component is essential for maintaining the fluidity of the cell membrane?
  • What is the role of insulin in the body?
  • When blood is filtered through the glomerulus, what is primarily retained in the bloodstream?
  • What is the connection between two neurons called?
  • What type of cell structure is primarily responsible for the movement of an entire cell?
  • Where does oxidative phosphorylation take place in eukaryotic cells?
  • Which of the following actions is NOT an example of a reflex action?
  • What is a mutagen?
  • How do the nervous system and the endocrine system work together?
  • Which enzyme is responsible for proofreading new nucleotide sequences for correctness?
  • What is a key characteristic of andropause?
  • What neurotransmitter is produced at neuromuscular junctions and synapses in the parasympathetic division of the nervous system?
  • How much ATP is produced from one glucose molecule before oxidative phosphorylation?
  • What is a function of the cerebral cortex on the surface of the parietal lobe?
  • What is the main function of bicarbonate ions in the kidneys during pH regulation?
  • What is hemodialysis primarily used for?
  • What are the structural and functional units of the kidney known as?
  • What is the main function of the adrenal glands?
  • What is the primary function of the sodium pump in cells?
  • What symptom is a result of the overproduction of hGH during childhood?
  • What occurs after DNA replication in terms of strand fidelity?
  • What does the term 'semi-conservative' refer to in DNA replication?
  • How does the structure of phospholipids contribute to their function in cell membranes?
  • What type of energy describes the potential to do work within a stored system?
  • What end product is formed in the Krebs cycle?
  • How can a functional group be defined?
  • What does the 5' cap signify for mRNA molecules?
  • Which cell types are the functional units of the nervous system?
  • What is helicase's primary function in DNA replication?
  • Which enzyme adds a short segment of RNA to initiate DNA replication?
  • Which structure serves as the filtration unit at the top of each nephron?
  • What effect is most likely to occur in plant cells when placed in a hypertonic solution?
  • Which of the following glands function exclusively as endocrine glands?
  • The redundancy of the genetic code primarily helps to explain what phenomenon?
  • What causes menstrual cycles to stop during pregnancy?
  • What impact does stress have on hormone levels in the body?
  • What is the monomer for nucleic acids?
  • What is the term for the process of separating wastes from body fluids and eliminating them from the body?
  • What is the primary method by which kidneys regulate body fluid balance?
  • What is the normal pH of blood?
  • What type of reaction does glycolysis represent?
  • What does gene expression refer to?
  • What distinguishes DNA from RNA in terms of sugar structure?
  • What are nitrogenous wastes?
  • What are the basic building blocks of DNA?
  • Which of the following is NOT a stage of DNA replication?
  • How do the lipid bonds in hydrogenated fats differ from those in natural fats?
  • Which cerebral lobe is involved with visual perception?
  • Which enzyme removes the RNA primer and replaces it with DNA nucleotides?
  • Which of the following processes is part of urine formation?
  • At the junction where one neuron transmits an impulse to another, what is this called?
  • In which organism is the lac operon primarily found?
  • What is the primary function of nephrons in the kidneys?
  • Thermodynamics primarily deals with which type of energy loss?
  • What is the meaning of a negative feedback loop in hormone regulation?
  • Which enzyme connects RNA nucleotides to a DNA template during transcription?
  • What is the primary function of DNA ligase?
  • Animal cells placed in a hypotonic solution are likely to...
  • What is chromatin primarily composed of?
  • What is the primary function of mRNA?
  • What does the cell membrane primarily consist of?
  • Which hormone is primarily involved in regulating the body's water balance?
  • During the formation of the replication fork, what is the main role of helicase?
  • What is the primary effect of thyroxine?
  • What is the main function of oxygen in oxidative phosphorylation?
  • Which statement best describes coupled reactions?
  • Which type of molecule is mRNA classified as?
  • How does cortisol function in the body?
  • During which phase can a neuronal membrane not be stimulated to undergo an action potential?
  • Which structure within the chloroplast is directly involved in capturing light energy?
  • What occurs during transcription in gene expression?
  • What effect do sex hormones have on the body?
  • How is kinetic energy distinct from potential energy?
  • During which transcription step does RNA polymerase move along the DNA?
  • Which of the following describes the outcome of glycolysis?
  • What type of therapy involves the use of an external device to filter blood?
  • Which type of neuron is characterized by a long axon and many shorter dendrites?
  • A chemical reaction will release energy if it is classified as?
  • Which organelle is known as the powerhouse of the cell?
  • What is the primary function of carbohydrates in biological systems?
  • What is the function of the antisense strand during transcription?
  • What is the role of an electron carrier in cellular metabolism?
  • Which lobe of the cerebrum is involved in interpretation and understanding of speech?
  • Which of these is NOT normally found in urine?
  • What term describes the matter formed by myelinated neurons in the central nervous system?
  • What does the term electronegativity refer to?
  • What is the primary function of ribosomes within a cell?
  • What is the primary function of the smooth endoplasmic reticulum?
  • How is urine transported from the kidneys to the bladder?
  • What process involves the removal of intron sequences and joining of exons?
  • Which part of the tRNA interacts with the mRNA during translation?
  • Why are radioisotopes considered important in Canada?
  • Which organ has sphincters that relax to allow urine to drain into the urethra?
  • What is the role of the promoter region in DNA?
  • How do single-strand-binding proteins assist in DNA replication?
  • Which of the following are functions of the cerebrum?
  • What does complementary base pairing refer to in nucleic acids?
  • What term is used to describe the condition when the membrane potential becomes slightly more negative than its original resting potential?
  • What is the main function of the collecting duct in the kidneys?
  • Which of the following accurately describes kidney stones?
  • Which component of the cell structure is crucial for maintaining shape and organizing the cell?
  • What is the primary purpose of the renal cortex?
  • Which of the following conditions may indicate renal failure?
  • Which hormone is often affected by kidney dysfunction?
  • What is released as a byproduct of the electron transport chain during aerobic respiration?
  • What is the main role of tRNA in translation?
  • What structure serves as a bundle of white matter linking the cerebral hemispheres?
  • What term describes the process of adding a phosphate group to glucose?
  • Which function is NOT associated with proteins in the cell membrane?
  • What is the ultimate function of a promoter region on a chromosome?
  • Which organelle is primarily responsible for protein synthesis?
  • What is the typical age range for the onset of menopause in human females?
  • What do chloroplasts primarily do in plant cells?
  • Which organ is NOT part of the excretory system?
  • What substance is produced when athletes push their cells to use up O2?
  • Which enzyme is tasked with adding nucleotides during DNA chain elongation?
  • What phenomenon describes when multiple codons correspond to the same amino acid?
  • How are nucleotides linked together in DNA?
  • According to the triplet hypothesis, what is the relationship between nucleotides and amino acids?
  • What mechanism do the kidneys use to manage blood pH levels?
  • Which of these factors would decrease the production of antidiuretic hormone (ADH)?
  • Which hormone released from the hypothalamus increases when puberty begins?
  • In what way do anabolic pathways differ from catabolic pathways?
  • Why is the brain considered the real sense organ?
  • What is the role of transcription factors in the process of transcription?
  • What is the liquid waste product that exits the body through the urethra?
  • What is the total ATP yield from complete eukaryotic aerobic respiration of one glucose molecule?
  • Which strand is transcribed and ultimately used in translation?
  • Which hormone is primarily responsible for the maturation of follicles?
  • Gene regulation controls which aspect of genetics?
  • What does mispairing during DNA replication involve?
  • What is the purpose of hormone replacement therapy (HRT)?
  • What is the process of creating a new strand of DNA called?
  • Which term does NOT apply to the structure of chloroplasts?
  • Which of the following is NOT a process of cellular respiration?
  • Which hormone is produced by the adrenal cortex as a response to long-term stress?
  • What is the primary function of RNA polymerase in gene expression?
  • Where is ammonia converted to the less toxic compound known as urea?
  • What is the role of catalysts in biochemical reactions?
  • Which type of substances are typically unable to pass through the glomerulus during filtration?
  • What primary effect does inhibin have on the anterior pituitary gland?
  • What constitutes the endocrine system?
  • What chemical messenger promotes changes such as the formation of facial hair?
  • What is the function of single-strand-binding proteins during DNA replication?
  • Which of these is an example of potential energy?
  • What is the role of the replication fork during DNA replication?
  • What is the process of producing an RNA copy from a gene called?
  • A nucleotide is comprised of which of the following?
  • Which component is found exclusively in DNA?
  • Which components are required for translation to occur?
  • Which statement best describes the structure of DNA?
  • What is the term for the occurrence when a stimulus causes the axon membrane to depolarize to a certain level?
  • What is the role of snRNA in cells?
  • Which structure connects the left and right cerebral hemispheres?
  • Which region of the kidney is the outermost layer?
  • What characterizes a telomere?
  • What liquid circulates throughout the spaces within the brain and spinal cord?
  • What are the three layers of tough elastic tissue that enclose the brain and spinal cord called?
  • In human males, what organ of the excretory system merges with the vas deferens of the reproductive tract?
  • In the ribosome, where does the tRNA with the amino acid enter?
  • How does the kidney respond to an increase in blood acidity?
  • Which of the following is a function of a restriction endonuclease?
  • What is the name of the pipe-like structure in the kidney that collects urine?
  • What macromolecules make up chromosomes?
  • What term describes the period in a woman's life when the menstrual cycle ends?
  • Which of the following describes intermediate filaments?
  • In which part of the cell does oxidative phosphorylation primarily occur?
  • Which enzyme catalyzes the addition of new nucleotides during DNA synthesis?
  • What is formed on the lagging strand during DNA replication?
  • DNA polymerase I is primarily responsible for which action during DNA replication?
  • Which structural feature in cells is primarily involved in intracellular support?
  • Where does the amino acid attach itself on the tRNA molecule?
  • What is the thin outer covering of grey matter covering each cerebral hemisphere of the brain known as?
  • Where does protein production occur in relation to DNA?
  • What are the four main processes that occur during urine formation?
  • In which part of the body is hemodialysis typically performed?
  • Which organelle is responsible for converting high-energy organic molecules into usable energy?
  • In what direction does DNA elongation occur during replication?
  • The role of the collecting duct in the kidneys is primarily for?
  • What is free energy?
  • An exergonic reaction is characterized by what type of energy change?
  • Which system is primarily responsible for the regulation of the body's excretion of waste?
  • What is an example of a positive feedback system in homeostasis?
  • What is the role of DNA ligase in DNA replication?
  • What protective barrier separates the blood from the central nervous system (CNS)?
  • Why is ATP considered the energy currency of the cell?
  • What is the fundamental unit of proteins?
  • What does the term metabolism encompass?
  • What determines which amino acid is attached to the tRNA?
  • How can neurons be classified?
  • What is the product of translation in gene expression?
  • In what form is the primary waste product of protein metabolism excreted by the kidneys?
  • What is the function of a flagellum?
  • What process occurs in the Bowman's capsule?
  • Which molecule carries the genetic information necessary for protein synthesis?
  • Where is the codon found in the process of protein synthesis?
  • What occurs during the denaturation of a protein?
  • What does it mean for the genetic code to be continuous?
  • Renal insufficiency primarily affects which body system?
  • What is the significance of a silent mutation?
  • What is defined as energy from a chemical reaction that is available for doing work?
  • How many ATP molecules are produced during the process of glycolysis?
  • What type of biological molecule is chlorophyll?
  • Which component is primarily responsible for carrying genetic information during transcription?
  • What is the composition of microfilaments?
  • In what way are DNA and RNA similar?
  • Where are the islets of Langerhans located?
  • Which gland is responsible for regulating metabolism?
  • In which organ is estrogen primarily produced?
  • How does a gene differ from a genome?
  • The hydrolysis of ATP is dependent on the presence of?
  • How is carbon dioxide primarily removed from a cell?
  • What is the functional unit of the kidney?
  • What is the direct cause of fatigue and lack of energy due to insufficient insulin production?
  • What is the role of macrophages in the human immune system?
  • Which component of the filtrate primarily contributes to the decrease in volume as it becomes urine?
  • What is the term for the organs that are responsible for producing reproductive cells?
  • Which of the following is an example of an intermolecular force?
  • What is the role of gonadotropin-releasing hormone (GnRH)?
  • What is the term used to define the gradual decline in testosterone levels in a man's life?
  • What is a spontaneous mutation?
  • What is one characteristic that makes RNA different from DNA?
  • When does a neural impulse begin to travel along a myelinated neuron?
  • What molecule serves as the template for protein synthesis?
  • Which of the following is NOT a nitrogenous base found in DNA?
  • What does it mean to be lactose-intolerant?
  • Which organs are part of the excretory system?
  • What are chemical messengers that carry signals from one neuron to another called?
  • In DNA replication, what purpose do RNA primers serve?
  • How does reabsorption of water occur in the nephrons?
  • What is the function of the acid-base buffer system in the body?
  • What role does the renal pelvis serve in the excretory system?
  • What happens during hyperpolarization of a neuron?
  • What is the function of the anticodon loop in tRNA?
  • What type of bond is primarily responsible for the structure of DNA?
  • How do hormones affect specific tissues or organs in the body?
  • Which product of the Krebs cycle does NOT contribute to energy production in cellular respiration?
  • What structure directs blood into the glomerulus?
  • Which hormone inhibits the production of FSH by the anterior pituitary?
  • Which neurotransmitter is the only one used by the parasympathetic nervous system?
  • Where is the anticodon located?
  • Where does Acetyl-coenzyme A enter during cellular metabolism?
  • What term is used to describe the process of reducing a membrane's potential from resting state?
  • What hormone stimulates the production of reproductive cells in the gonads?
  • The process by which excess substances are removed from the blood into the nephron is called?
  • Which molecule is crucial for transporting oxygen in the blood?
  • Isomers differ primarily in what aspect?
  • What is pyruvate primarily characterized as in cellular respiration?
  • What are cilia best known for?
  • A lack of iodine in the diet may lead to which disorder?
  • What is the role of follicle-stimulating hormone (FSH) in the reproductive system?
  • Which of the following is true about ATP production through cellular respiration?
  • How do cells generally repair mutations?
  • What is a primary chemical messenger produced by the ovaries?
  • How do insulin and glucagon regulate blood glucose levels?
  • Which of the following best describes the primary role of enzymes in biological reactions?
  • What role does chemiosmosis play in cellular respiration?
  • Where are neurotransmitters released from?
  • What is the role of topoisomerase II during DNA replication?
  • What is the main function of the corpus callosum?
  • How can a neural impulse in an unmyelinated fiber be described?
  • Which blood vessel carries blood from the aorta to the kidneys?
  • What is the function of restriction endonuclease in recombinant DNA technology?
  • Which response is not found in the short-term stress response?
  • Which of the following bases pairs with adenine in DNA?
  • Which blood vessel drains blood from the kidney to the body?
  • Which of the following describes a key characteristic of anabolic pathways?
  • What distinguishes isotopes of an element?
  • What best describes the relationship between the two DNA strands at the replication fork?
  • Which term describes the process of urine formation in the kidneys?
  • Which term describes a chemical reaction that requires energy input?
  • What type of bond is formed between amino acids in a polypeptide chain?
  • What organ temporarily stores urine before it is eliminated from the body?
  • What is the role of the poly-A tail in RNA?
  • What is the major function of ribosomes in cells?
  • What distinguishes lipids from carbohydrates?
  • Which term describes the process of compacting DNA?
  • Activation energy can be reduced by the presence of?
  • Which of the following best describes the function of helicase?
  • What is the last structure in the sequence of urine flow?
  • Which disorder is best treated with medication containing thyroxine?
  • What role do ribosomes play in translation?
  • Which of the following is NOT a disorder of the excretory system?
  • Which structure is characterized as forming hollow tubes within the cell?
  • What unique feature is found in the structure of mitochondria?
  • Which of the following is NOT a function of the excretory system?
  • Which hormone is classified as a lipid?
  • How is energy best defined in a biological context?
  • Which step of transcription is responsible for selecting the correct start site?
  • What are chemical messengers that control the development and function of the reproductive system known as?
  • Which hormone plays a crucial role in the fight-or-flight response?
  • What characterizes the renal medulla in the kidney?
  • What best describes metabolic pathways?
  • What is a synapse?
  • During replication, which enzyme unwinds the DNA double helix?
  • Facilitated diffusion differs from simple diffusion in that...
  • What is an example of a negative feedback system in homeostasis?
  • Which enzyme is responsible for unwinding the DNA helix during replication?
  • What initiates the process of transcription?
  • What does it mean for the genetic code to be redundant?
  • How does Co-enzyme A assist in the Krebs cycle?
  • Which of the following codons is recognized as the START codon in protein synthesis?
  • What is the process called when multiple identical copies of a gene are created?
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